What are the advantages or disadvantages of sonication over chemical procedures?
In most instances the sonication technique, which takes only 30 min from start to finish, can rapidly extract fresh, frozen, or formalin-fixed paraffin-embedded tissue and is superior to other rapid extraction techniques in terms of quality and quantity of DNA. It is much more rapid than those techniques that use long digestion periods. This technique will be of great value to those investigators extracting DNA for polymerase chain reaction assays.
In most instances the sonication technique, which takes only 30 min from start to finish, can rapidly extract fresh, frozen, or formalin-fixed paraffin-embedded tissue and is superior to other rapid extraction techniques in terms of quality and quantity of DNA. It is much more rapid than those techniques that use long digestion periods. This technique will be of great value to those investigators extracting DNA for polymerase chain reaction assays.
Hi,
The answer to your question depends on the source of your DNA.
There would be a difference between eukaryote, bacterial or plant cells, and also between cultured cells or tissue samples (I would not recommend sonicating tissues).
Sonication can create DNA strand breaks, but on the other hand it is pretty fast, of minimal additional cost per sample (if you already have a Sonicator) and does not require use of toxic reagents such as Phenol and Chloroform (used extensively).
You should probably consider also what you want to do with your DNA after extraction, since this could also have ramification.
Also, there are two types of sonicator devices: needle and bath.
Which are you planning to use?
The first would be better for higher volume samples (in general); the second is gentler but can simultaneously sonicate many samples and also it is better for sterility.
I need to extract genomic dna from yeast cells what frequency is better and how much time it needs and can i use phenol chloroform after sonication to get a good yield?
In comparison with the other methods like Freeze thaw, Chemical lysis, Cell Bomb and bead mill/ ball mill, Sonification Method is very efficient and reliable method that allows the complete control over the sonification parameters. The best thing is product purity. Easily applicable and suitable for all cell types.
Hi can i ask what are the advantages and disadvantage of using phenol method of extraction RNA
- Badges
- Science topic
- Similar topics
- Cell Biology
- Methods