Does all mineral oil contains asphaltenes?

I had a mineral oil of 460cSt (containing sulphur and phosphorus), after addition of nanparticles (carbon nano tubes and graphene) the viscosity decreased significantly. literatures says that the...

16 August 2018 6,476 1 View

Which is more preferable mixed lubrication regime or boundary lubrication regime?

04 July 2016 1,862 6 View

How does Young's modulus,shear modulus,Poisson ratio of a nano wire/tube/particle influence the coefficient of friction?

Mechanical properties influencing coefficnet of friction.

21 June 2016 1,344 2 View

Can we get lower coefficient of friction with larger scar diameter in a Four Ball Test rig?

Two tests were carried out. One with a vegetable oil and the other with mineral oil. The mineral oil viscosity was almost twice than the vegetable oil. It was observed that the Coefficient of...

10 January 2016 8,250 6 View

I performed an experiment and the following were negative outputs in the response table. Why?

An L9 orthogonal DOE was performed using Minitab. The S/N in case of frictional force is negative.The response of the frictional force and rpm were negative. But in case of other output...

10 July 2015 6,731 5 View

While analyzing a pin-on-disc experiment using Taguchi analysis which type of problem has to be considered- static or dynamic?

21 March 2015 3,441 2 View

In pin on disk how are you calculating the total wear track distance?

Formula.

12 May 2014 4,071 10 View

When we add solid particles to a liquid what can we call them: emulsions or dispersions?

Addition of additives in lubricant is to be called what?

06 May 2014 6,107 9 View

What is the main reason that nano particles get agglomerated?

Nano particles such as copper oxide, zinc oxide and carbon nano tubes.

23 March 2014 9,249 40 View

What is the reduced radius when we use flat faced cylindrical pins for a pin-on-disk experiment?

In hertzian models we have models for spherical -spherical and spherical-flat, but flat-flat surface is not shown.

13 December 2013 7,749 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View