Sangeet Bhaumik

6 Questions 9 Answers 0 Followers

Questions related from Sangeet Bhaumik

PCR inhibitor: Isopropyl acetate (2.5%)?

If extracted viral RNA contains 2.5% isopropyl acetate, would it be acting as PCR inhibitor?

21 October 2020 3,152 3 View

How to normalize the fluorescent data obtained from spectrofluorometer?

I have a target gene, which is differentially expressed in lung cancer patient, house keeping gene, and a third gene which is highly expressed in all lung cancer patients. I took the fluorescent...

15 April 2016 8,230 3 View

What are the excitation spectra of N-Methyl mesoporphyrin in a flurometer?

In my current study, I am using NMM as fluorescence material. Most of the previous study mentioned the excitation spectra of NMM is 399nm and the emission spectra is 610nm. Is it possible that I...

03 July 2015 3,649 4 View

Can I use Qubit 2.0 (Invitrogen) fluorometer in place of F-7000 spectrometer (Hitachi, Japan)?

Yan et al., 2014. in their paper "A G-quadruplex DNA-based, Label-Free and Ultra sensitive Strategy for microRNA Detection" (paper is attached with this post) suggested a ultra sensitive method...

28 April 2015 7,380 1 View

What are the advantages or disadvantages of DNA extraction by sonication?

What are the advantages or disadvantages of sonication over chemical procedures?

17 September 2013 7,286 8 View

How do you use the helical wheel diagram?

I was analyzing BRAF sequence by BioEdit software and accidentally I have came across this "helical wheel diagram" made by the software. Though it is very interesting, I have no idea about it. Can...

26 August 2013 1,841 2 View