What are some stripping protocols that are effective for PVDF western blot membranes with Licor secondaries?

16 April 2025 1 7K Report

I have used previously the Licor's NewBlot PVDF 5x Stripping Buffer with 0.5% (v/v) SDS for 20 and 60 minutes, however, the primary and secondary antibodies are not being removed.

Yan Cui

We use 10% acetic acid as a general stripping solution, which can remove ~90% of antibodies within 5-15 min from probed NC membranes and does not cause loss of blotted proteins. We have not tested with PVDF membranes, but we expect a similar efficacy. See Article Combining Acid Stripping With Photobleaching for Sequential ...

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