What are some commonly used glioblastoma (GBM) cell lines that people use?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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Nathan Haigh

Hi James, a compatriot uses U-87 MG, U-118 MG, and T98G as glioblastoma cell lines in his lab. These should all be commercially available, from ATCC for instance.

James P. Madigan

Thank you Nathan!

Carsten Schmidt

Hi James,

also C6, a rat glial tumor cell line, is often used.

Carsten

Carsten,

Thank you! I actually have rat-specific CRISPR for my gene/protein of interest. I will look into this line also. Again, thank you!