James P. Madigan

5 Questions 19 Answers 0 Followers

Questions related from James P. Madigan

How transfectable are U87MG cells?

I was wondering if someone might have experience transfecting expression plasmids into U87MG (glioblastoma) cells? I would like to transfect in an expression plasmid and overexpress a protein in...

06 June 2018 466 1 View

How "transfectable" are mouse embryo fibroblasts (MEFs)?

I have some +/+ and -/- MEFs of a certain gene and want to add back in WT and various mutant cDNA of this gene, to determine functionality. I was planning to transfect the various cDNA's/plasmids...

05 May 2017 6,040 3 View

What are some commonly used glioblastoma (GBM) cell lines that people use?

I would like to do some signaling studies in GBM cell lines. Might someone suggest some commonly used ones? Thank you!

05 May 2016 7,067 4 View

Has anyone used a colorometric or fluorometric kit to measure free fatty acids in cell culture cells?

I want to measure possible changes in fatty acids in various cancer cells in culture after modulating the expression of a certain protein? I don't have the $ or the resources to do this...

05 May 2016 686 1 View

Is there an upper limit to the size of a protein that can be expressed in BL-21 bacteria?

I understand every protein is different regarding induction and solubility. I'm just wondering if there is a rule of thumb regarding a molecular weight limit. I'm thinking about trying to express...

12 December 2014 1,470 7 View