suspention buffer containTris (pH 7.5), and EDTA (ethylenediaminetetraacetic acid).The basic pH helps to denature the DNA and the metal ion chelator, EDTA, stabilizes

the cell membrane by binding the divalent cations of Mg2+ and Ca2+. and lysis buffer contains sodium hydroxide and SDS (sodium dodecyl sulfate). The sodium hydroxide denatures the plasmid and chromosomal DNA into single strands. SDS, an ionic (charged) detergent dissolves the phospholipids in the membrane causing lysis and release of the bacteriacontents, including the DNA, into the solution.

you added lysis buffer instead of Resuspention buffer. so first your suspention was not carried out because cell membrane was not stabilized and DNA was not denatured but it came out into solution. phospholipids of cell membrane dissolved by SDS of lysis buffer. next you can think what will happen if you continue do this......

Hey i want to know the component of lysis viewer provided with the plasmid isolation kit. I mistakenly added lysis viewer not lysis buffer.

Hi Sahil,  from few web research I only find a site where  the "lysis viewer" was indentify as "Lysis & Neutralization indicator", I remembered using the "Qiagen Lysis Blue" in this way. If this is your case, but I can't be sure, this reagent works as an pH indicator that the lysis and neutralization reactions occured, and occured in the correct way. In general, you add this reagent to lysis and neutralization buffers not to the resuspension one, so it is seemed you don't make a mistake.

Anyway, I think would be better if you find your plasmid isolation kit "datasheet" or ask to your coworkers!!!