Sahil Sharma

13 Questions 26 Answers 0 Followers

Questions related from Sahil Sharma

Why do I get an error while doing mutation pcr in a g rich region using PFU?

I am doing mutation pcr using quickchange mutation. While using pfu which has proof reading activity, I am getting wrong mutated product for example getting more g or duplication or mutation at...

30 December 2015 6,839 3 View

30 percent denaturing urea Gel Electrophoresis protocol for running in 15 cm long gel?

I want to run a 20 bp sequencing reaction in 15 cm long gel. Is it possible to run this by making a higher percentage of acylamide. Until now I am not getting a fully resolved band.

04 December 2015 8,796 6 View

How can I chose a cell line for luciferase reporter construct experiment?

I have cloned promotor of a gene upstream of luciferase gene. I have seen people using different cell lines in the papers for dual luciferase experiment.Can I just use hek cell line to see the...

19 August 2015 850 2 View

How to visualise and excise radiolabelled dna band at same time from native polyacrylamide gel?

I have two questions.I have problem in making 16percent native polyacylamide gel and16% percent urea-denaturing gel. Gel doesnt polymerise properly.What composition of components should I use for...

08 August 2015 2,621 9 View

Does anyone has a working protocol for DNA polymerase stop assay and DMS footprinting for G quadruplex?

I want to perform dna polymerase stop assay and DMS footprinting for my g quadruplex forming oligo.Our lab is new to this topic and no one in our lab has experienced about this.I have tried...

07 August 2015 8,351 5 View

Is there any website where I can know the chemical name by giving structure in pdf file?

I was reading a thesis in which i encounter the compounds that the author used for his experiment.He provides the structure of all 10 compunds but not name.I have attached the pdf file.please have...

23 July 2015 8,940 9 View

Any advice on a DNA polymerase stop assay for g quadruplex?

I want to perform dna polymerase stop assay for my g quadruplex forming oligo.Does anyone has a good protocol for that. I have a protocol but I am not abe to understand following parts- {Prepare...">

19 June 2015 4,047 1 View

What are components of lysis viewer in plasmid isolation kit?

I mistakenly added lysis viewer to lysis buffer instead of resuspension buffer.Can i use the lysis buffer again.How lysis viewer works.

07 April 2015 3,185 3 View

Any commercial company that sells iron core gold shell nano-particles?

In my lab first we tried to make gold iron nanoparticles but nanoparticles always get aggregated.Now i am looking for a company who sells nanoparticles having iron core with gold shell but could`t...

02 April 2015 3,080 8 View

How can I calculate Gold nanoparticle concentration in Molars and coverage?

I have some doubts.hope u guys can clear. I am attaching thioated dna with gold nanoparticle. I know my thiolated dna concentration in molars but I don`t know how to calculate gold nanoparticle...

30 March 2015 2,439 6 View

inhbitors or antagonists of cationic porphyrins like Tmpyp4 in G quadraplex study?

I am working on various G rich sequences which can form g quadraplex. My G rich oligo sequence is making g quadraplex and it is getting destabilized by cationic porphyrins like Tmpyp4 (CD data)....

29 March 2015 7,665 4 View

How can I coat gold nanoparticles?

I am working with using gold nanoparticles to find sequence specific proteins. I attached the thiolated sequence specific oligo (sequence specific to one DNA binding protein) to gold nanoparticle...

22 March 2015 2,246 1 View

I need a protocol to coat gold nanoparticle so that it will not react to other proteins?

I am working with using gold nanoparticles to find sequence specific proteins.For control, I attached the thiolated sequence specific oligo (sequence specific to one dna binding protein) to gold...

20 March 2015 9,630 6 View