Please say whatever analysis can we do in wild legumes except the following
1. proximate composition
2. mineral composition
3. vitamin analysis
4. amino acid profile
5. fatty acid analysis
6. seed protein fractionation
7.antinutritional factors
8. antioxidant activity
Starch quality analysis: amylose and amylopectin content, chain length analysis, Rapid Visco Analyzer pasting properties, Differential Scanning Calorimetry, X-ray diffraction (the latter unlikely to bring any surprises), granule size distribution, granule porometry.
Protein extractability: for milks, yoghurts, tofu and other foods.
Sterols (related to your lipid analyses and vitamin analyses, but not included in either).
The wild legume seeds can be grown and compared for various morphological characters. Any best observed character can be transferred to the cultivated varieties for improvement. The problems like shattering, growth habit and disease resistance can be addressed by looking at the genes present in the wild species by growing their seeds. Seeds can also be used to study the mitosis. The chromosome numbers can be used to understand the phylogenetic relationship, cytotypes, polyploids or aneuploids if there is any.
Invitro antidiabetic assays and invivo trial (rat, Fish and poultry) - for protein replacement. Invitro protein and starch digestibility, resistant starch. Protein fractionation.
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