What amount of raw reads (in million reads) per sample do I need for a differential miRNA epressional analysis with the Illumina HiSeq2000 platform?

05 May 2016 0 3K Report

I am planning to run mouse tissue samples for sequencing, with the objective to see differential expression of known miRNAs... What should be the minimum amount of reads per sample, that would suffice for such an analysis?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

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How to confirm the site-directed mutagenesis result without performing NGS?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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Why activated CAR-Jurkat cell could not kill targets?

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Anyone having idea about VN primer for miRNA primer design ?

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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