What Allele Specific PCR Primer designing tool can you suggest?

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Where to perform invitro transcription and capping?

I am planning to do invitro transcription and capping for an mRNA vaccine generation. Can anyone suggest whether it should be done on a bench top or inside a laminar hood?

14 May 2024 186 3 View

Does the absolute quantum yield of a fluorophore material change with change in the concentration in the solution?

Recently, I synthesized a fluorophore that shows aggregation in the solid state. I measured the absolute quantum yield in different solvents and observed different results. At the same time, while...

02 February 2024 7,617 1 View

What should be the degassing temperature and time of a porous organic cage material for the BET analysis?

The porous organic cage material was synthesized in a biphasic solvent mixture (chloroform + methanol).

29 January 2024 3,829 2 View

Why should researchers review journal articles for free when access to journal articles is not free?

Often researchers get articles to review and they are paid nothing. The same publisher charge for accessing the journal articles. Doesn't it seems nonsense? Expecting your view in this regard.

01 October 2023 640 18 View

What should be done if the modelled protein structure fails to pass VERIFY3D?

After refining the protein structure multiple times, it still fails to pass VERIFY3D. The ERRAT score is 96.9298 and PROCHECK results are fine. What can be done to improve the VERIFY3D results?

23 April 2023 6,761 3 View

What is the precise threshold concentration (in measurable units) of QS signaling molecules?

Bacterial Quorum Sensing relies on the synthesis, secretion, and detection of autoinducers to regulate gene expression in response to cell density. The small chemical signals are synthesized...

27 February 2023 2,750 0 View

What is the difference between hydraulic retention time and operating time in a wastewater treatment reactor?

Hydraulic retention time (HRT) is the average time a certain amount of wastewater stays in a holding tank before exiting the tank. But then what is the operating time?

19 January 2023 921 5 View

Problem in protein structure prediction using AlphaFold and subsequent docking using Autodock?

After the protein structure prediction using AlphaFold2, I want to proceed with molecular docking in Autodock. However, the modelled protein structure cannot be opened using Autodock with the...

03 January 2023 8,608 10 View

Can we evaluate EPS-protein and metal interaction using docking?

Biofilm bacteria show increased resistance to toxic heavy metals. The remarkable tolerance is often attributed to the EPS matrix. Several studies have confirmed the involvement of protein like...

17 February 2022 9,599 0 View

What is the difference between GC-MS and HRMS?

What is the difference between GC-MS and HRMS? Which is better for identification of compounds in a crude extract (mixture of compounds)?

12 July 2021 538 6 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View