Recently, I synthesized a fluorophore that shows aggregation in the solid state. I measured the absolute quantum yield in different solvents and observed different results. At the same time, while varying the concentration in a solvent, the absolute quantum changed. Did I get the correct result, or should I measure it with a different instrument?
The absolute quantum yield of a fluorophore material typically remains constant with changes in concentration within a solution, assuming other conditions like temperature and solvent remain consistent. However, in some cases, there might be concentration-dependent quenching or aggregation effects that could affect fluorescence properties.
The observed changes in absolute quantum yield with varying concentration and solvent suggest potential aggregation effects, which can influence fluorescence properties. Consider using different techniques or instruments to validate your results, such as steady-state and time-resolved fluorescence spectroscopy. Additionally, ensuring proper sample preparation and accounting for any artifacts in measurements can enhance result accuracy.