Is there any kind of interference between wireless networks?

during my day to day I could appreciate how the intensity of the wifi networks are altered because of the existence of other networks, here is my question because I want to know if. Is there any...

05 July 2019 5,007 5 View

Why the sewage water I treated using electrocoagulation & iron electrodes resulted to have a very intense yellow color and unstable sludge?

I've already adjusted the pH of my sewage sample between 2-12 but still the result is a water with rust like color. I've already considered changing the current supply and electrolysis time but...

06 April 2018 6,464 3 View

What are the challenges of Regenerating/reviving a dead cell, using bioelectronics or other methods.

dormant cells don't undergo any metabolic processes, but an appropriate stimuli can activate the Partially dead cells, to fully functional cells. Regenerating/reviving a dead cell, using...

10 July 2017 8,810 1 View

What is the reason for see two bands in PCR of genomic DNA of pichia x-33 strain?

07 June 2016 3,262 1 View

How is the best way to disperse diamond nanopowder (<10 nm) in water?

Hi everyone! I would like to disperse 10 nm diamond powder (Sigma-Aldrich) in deionized water without any dispersant. Is that possible? If not, I could allow for some dispersant, but which...

30 May 2016 4,116 5 View

How much DNA template (synthetic gene) is used for a general PCR?

22 May 2016 7,659 11 View

Selecting pichia phenotype?

Hi everybody, I am trying to determine the phenotype of my transformation of pichia, I have the strains, X33 and SMD1168H, I did changes in the recommendations of the protocol of easy select...

11 April 2016 4,985 1 View

Is there a phosphate glass which has homogeneous bulk nucleation?

Silicates and borates glasses, for example, there are several compositions which exhibit homogeneous bulk nucleation, however this does not occur aparentement for phosphates.

08 April 2013 4,052 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View