I will be working with thermophiles, and I want to culture them on solid media, and view colonies. However, traditional agar melts at lower temperatures, and my desired temperature is 80 °C
Hi, I do not have personal experience with it but I have often seen Gellan Gum as the one that is recommended for this purpose.
Methods are based on Chel-Guerrero and colleagues'Phytochemical Profile, Toxicity, and Pharmacological Potenti... . We use a 1M Tris buffer with pH of 6.8, with Bovine Serum Albumin as the...
25 April 2024 8,802 0 View
I synthesized hydroxyapatite from an agroindustrial waste using the wet precipitation method. However, after synthesis, my material is still basic after several wash with deionised water and...
26 January 2022 7,766 5 View
Bone tissue engineering
04 July 2021 1,387 4 View
Be A(u) = ( u1 * A1 ) + ( u2 * A2 ) + ( u3 * A3 ), u = [ u1 u2 u3 ]T, Ai are known 3x3 real matrices, i=1,2,3. How to solve the following equation: Find the scalar \lambda and the vector u such...
08 March 2021 2,257 6 View
La premezcla sera de aire con propano
30 May 2020 1,557 0 View
during my day to day I could appreciate how the intensity of the wifi networks are altered because of the existence of other networks, here is my question because I want to know if. Is there any...
05 July 2019 5,167 5 View
I'm referring to the overlapping sigmoidal curves that are used to describe the gain-of-function mechanisms. I understand the activation curve, but I can't seem to get my head around the...
08 March 2019 1,584 3 View
I am modeling with gravimetry a small section of the rapakivi granite of Parguaza, Venezuela. I am located near from the left flanc of the Parguaza graben so I am also in an extensional setting....
09 September 2018 9,578 8 View
I have a cheap current/charge meter, kcx-017, to measure the full discharge of a battery connected to a raspberry pi. I intend to reduce the current delivered to the raspberry turning off some...
09 September 2018 1,916 3 View
I've already adjusted the pH of my sewage sample between 2-12 but still the result is a water with rust like color. I've already considered changing the current supply and electrolysis time but...
06 April 2018 6,568 3 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...
12 July 2024 3,756 4 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
Get too many colonies, which are mostly non-transformants when tested by colony PCR
11 July 2024 6,775 3 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?
10 July 2024 6,352 0 View
Hi. I have brightfield images of stem cells. I need to count the total number of cells/image. Some cells are individual and some cells are in colonies. Using average cross-sectional area of...
05 July 2024 4,197 1 View