I will study calcium flux in T cells using fluorescence, so I`ll need cells to stick to a plastic 96-well plate, without any spontaneous triggering of calcium currents.
Which substratum is best for T cell adhesion in this case?
Do you have to use the plate assay? Some cell types can get activated during adhesion or change its metabolism (affecting your result). I personally prefer flow cytometry for Calcium influx.
You could try poly L lysine, Sigma sells pre coated 96 well plates ready to use but I believe you can do it yourself at your lab. I've used for Microscopy (with coverslip) and it's very straightfoward.