Wether it is possible to dissolve Amyloid beta 1-40 monomer with phosphate buffer or do we need to HFIP?

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Saranya Sivaraj

Hi Sandhya, I think you need HFIP because I did see some particles settling at the bottom when dissolved using phosphate buffer.

Mahesh Chandak

It is not possible to use Phosphate buffer to dissolve amyloids . HFIP/DMSO. Alternative is dissolve in small amount of DMSO and then dilute it in PBS (Some people dont prefer DMSO )

Sandhya Aasuri

Thank you Saranya Sivaraj and Mahesh Chandak for your answer