Dear eminent researchers iam planning to start IHC study in ameloblastoma using FAP ALPHA. Need your opinion?

Fibroblast activation protein (FAP )alpha

28 December 2020 8,101 0 View

Which is the best machine learning method for topic extraction from large document collections?

I want to know about different algorithm/method/inference to extract underlying topics/themes from documents/scientific article etc.

12 October 2020 5,911 3 View

The porous nature of the material should show up in the impedance behaviour in terms of inductance element?

The porous nature of the material should show up in the impedance behaviour in terms of inductance element. I do not see any value given for the inductance at the interface in these plots which...

01 October 2020 7,504 2 View

Is there any way to separate MoS2 QDs from MoS2 flakes?

I have tried to exfoliate MoS2 via liquid exfolaition method(using NMP as well as Ethanol/water mixture as solvent) . Always Iam getting MoS2 nanoflakes and MoS2 QDs. Is there any way to separate...

03 February 2020 6,771 1 View

I want to coat Ionic liquid over Fe3o4@Sio2. I have very concentrated only 0.19g of ionic liquid. Does anyone have idea how can i do it?

I have successfully coated the Fe304@Sio2@PPy( Polypyrrole) simply by taking 100mg Fe304@Sio2 ,suspended in 15 ml DI water then sonicate for 15 min then add 100 microlitre pyrrole. Solution was...

13 January 2020 8,206 5 View

Support vector machine or neural network, which techniques is better for image classification problem?

pattern classification is a popular problem area, there are different methods available to do it.

16 September 2019 3,549 3 View

How the sampling is done in cohort,case control and cross sectional design ?

how the sampling is done in cohort,case control and cross sectional design ?

14 August 2018 830 5 View

Data Required

Hello friends, I need to know how to get the data if you are researching on supply chain. I am looking for costs and other inventory data for retail chains of India. How to go about it?

25 May 2017 783 3 View

Instead of osmium tetroxide is there any other substance that can be used as a secondary fixative for electron microscopy of bacterial cell?

25 September 2016 9,374 4 View

Hi..Can I induce protein expression in pTZ57R/T vector using IPTG?

Hi..I used pTZ57R/T vector for cloning my gene of interest. Is it necessary to subclone the gene into an expression vector to see the protein expression ? can I induce the protein expression in...

17 April 2016 4,648 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View