Any localization sequence to target cell organelles?

12 March 2024 0 2K Report

I am looking for the paper or the persons who worked on cloning of short sequences targeting the different cellular organelles (vacuole, mitochondria, plasma membrane, septum) in plant pathogenic fungus.

More Sandhya Neupane's questions See All

Dear eminent researchers iam planning to start IHC study in ameloblastoma using FAP ALPHA. Need your opinion?

Fibroblast activation protein (FAP )alpha

28 December 2020 8,101 0 View

What is the significance of hysteretic energy in RC member?

How can we use the energy demand of beams and columns or other structural memebers in real fileld? What are the applications of energy based design method?

25 November 2020 7,433 1 View

Which is the best machine learning method for topic extraction from large document collections?

I want to know about different algorithm/method/inference to extract underlying topics/themes from documents/scientific article etc.

12 October 2020 5,911 3 View

The porous nature of the material should show up in the impedance behaviour in terms of inductance element?

The porous nature of the material should show up in the impedance behaviour in terms of inductance element. I do not see any value given for the inductance at the interface in these plots which...

01 October 2020 7,504 2 View

Could someone suggest good journal/article realted to energy dissipation through hysteretic behaviour?

Currently I am working on " Quantitative Assessment of dissipated energy from structural components correlation with deformation under seismic loads in low rise building using Perform 3D".

13 July 2020 3,330 2 View

Does anyone know whether NCI-H522 and NCI-H1437 lung cancer cell lines grow as xenograft in nude mice?

Please let me know whether any body in this forum has first-hand experience growing them in nude mice. I was thinking about injecting 5 million cells in 1: 1 ratio of Matrigel and PBS. Let me know.

07 February 2020 4,661 1 View

Is there any way to separate MoS2 QDs from MoS2 flakes?

I have tried to exfoliate MoS2 via liquid exfolaition method(using NMP as well as Ethanol/water mixture as solvent) . Always Iam getting MoS2 nanoflakes and MoS2 QDs. Is there any way to separate...

03 February 2020 6,771 1 View

What would be a suitable econometric method to evaluate socio-economic impact of social security ?

Research methodology

25 January 2020 7,278 2 View

I want to coat Ionic liquid over Fe3o4@Sio2. I have very concentrated only 0.19g of ionic liquid. Does anyone have idea how can i do it?

I have successfully coated the Fe304@Sio2@PPy( Polypyrrole) simply by taking 100mg Fe304@Sio2 ,suspended in 15 ml DI water then sonicate for 15 min then add 100 microlitre pyrrole. Solution was...

13 January 2020 8,206 5 View

Wether it is possible to dissolve Amyloid beta 1-40 monomer with phosphate buffer or do we need to HFIP?

I have Amyloid beta 1-40 monomer using which i need to perform biophysical characterisations.

16 September 2019 4,472 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

Could someone please provide me with a reliable differentiation protocol for IMR-32 NB cells?

Treatment with 10uM Retinoic acid seems to result in vacuolization and cell death

20 January 2021 4,013 2 View

Where did Mitoprot software go (download version)?

Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...

18 January 2021 4,368 3 View

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling?

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling? Any thoughts on applicable small molecules to...

06 January 2021 4,324 2 View

Why do I see many vacuoles on my cancer cells?

I am currently growing BICR31 cancer cells for conditioned media collection, and as I passaged it I have seen many bubbles/vacuoles - like structures. Cells were healthy during the first split...

05 January 2021 2,320 5 View

Is it correct to expect the same quantitative result between SDS-PAGE and BN-PAGE analysis performed on the same sample?

Please, can anyone please help me with this issue? By BN-PAGE and western blot on DDM-solubilized mitochondria, isolated from cultured cells, I found that in treated cells the amount of CV...

05 December 2020 7,696 2 View

How to prevent protein aggregation in gel filtration (size exclusion) column ?

I am currently trying to run solubilised mitochondria extracts from mammalian cells through a gel filtration column in order to separate proteins by size & visualise protein complexes. My...

29 November 2020 1,483 5 View

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

05 November 2020 8,841 3 View