My samples are not resolved properly in the upper portion of gel - particularly in the higher molecular weight range - above 50 kDA. The same samples were loaded onto 4 independent gels giving different patterns (only 2 gels on attached picture). Based on that I exclude sample degradation as a cause of visible distortion.
Does any of you have an idea as to the possible cause of this distorted migration? Have you ever seen a similar pattern?
Erman Kocak
Is that a picture of the gel or stained membrane (Ponceau?) If the latter I would suggest it is maybe caused by the blotting device i.e something wrong with transfer stack or electrodes
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Malgorzata Wessels née Pietrzykowska
No, no luck. It is stain free gel.
I think It is the air bubbles in the gel.
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