21 January 2019 5 3K Report

I'm looking for the cause of black dots within a Western blot band (see attached image). I don't see these dots in the background, but they show up where my protein is supposed to be.

The primary antibody is new and I made sure to spin down the secondary before use (usually doesn't produce bands like this). This membrane was previously stained and was stripped prior to current blot. Any ideas?

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