Joe Beil

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Questions related from Joe Beil

Is it better to push or pull liquid through microfluidics with a syringe pump?

I have a PDMS microfluidic device that I want to perfuse cells through (there are also adherent cells in the channels). Is there any difference if I use a syringe pump to push the cells through...

31 January 2021 4,253 3 View

Western blot speckled bands?

I'm looking for the cause of black dots within a Western blot band (see attached image). I don't see these dots in the background, but they show up where my protein is supposed to be. The...

21 January 2019 3,340 5 View

Western blot speckled bands?

I'm looking for the cause of black dots within a Western blot band (see attached image). I don't see these dots in the background, but they show up where my protein is supposed to be. The...

01 January 2019 8,722 6 View

How to manage doing IHC/IF staining with many samples?

How do people do IHC or IF on many tissue samples? Say you have animal/human tissues (not a tissue microarray) that you want to stain with a protein and DAPI and you have 50 tissues in a Group A...

05 May 2018 6,378 8 View

How to manage doing IHC/IF staining with many samples?

How do people do IHC or IF on many tissue samples? Say you have animal/human tissues (not a tissue microarray) that you want to stain with a protein and DAPI and you have 50 tissues in a Group A...

04 May 2018 9,616 7 View

What software does Lab on a Chip use for journal covers?

Does anyone know what software produces journal covers like these? http://pubs.rsc.org/en/journals/journalissues/lc#!issueid=lc016003&type=current&issnprint=1473-0197

10 October 2017 773 0 View

Western blot - load equal volumes?

I have a problem with unequal loading of protein in WB (my housekeeping gene varies considerably across conditions). Here's what I do: Cell lysis with RIPA buffer and inhibitors for 30 min on ice....

05 July 2017 4,712 13 View