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Questions related from Joe Beil
I have a PDMS microfluidic device that I want to perfuse cells through (there are also adherent cells in the channels). Is there any difference if I use a syringe pump to push the cells through...
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I'm looking for the cause of black dots within a Western blot band (see attached image). I don't see these dots in the background, but they show up where my protein is supposed to be. The...
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How do people do IHC or IF on many tissue samples? Say you have animal/human tissues (not a tissue microarray) that you want to stain with a protein and DAPI and you have 50 tissues in a Group A...
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Does anyone know what software produces journal covers like these? http://pubs.rsc.org/en/journals/journalissues/lc#!issueid=lc016003&type=current&issnprint=1473-0197
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I have a problem with unequal loading of protein in WB (my housekeeping gene varies considerably across conditions). Here's what I do: Cell lysis with RIPA buffer and inhibitors for 30 min on ice....
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I understand that if you have 3 mice and are generating data from microscopy images, you might want to average the data in such a way that you enter in 3 numbers to Prism for example. Should you...
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