Brian Bower

6 Questions 85 Answers 0 Followers

Questions related from Brian Bower

Are there good RT-PCR (qPCR) primers for measuring expression from pCAGEN/pCAG promoter?

I am assembling several pCAGEN vectors, and I want to design a set of primers to measure expression of mRNA from the pCAG promoter. My initial strategy has been to design primers flanking the...

25 October 2016 1,878 1 View

How transferable are FPLC skills to HPLC?

I'm job hunting these days, and I have a lot of marketable experience using fast protein liquid chromatography (FPLC) for protein purification. However, many positions I'm applying to also prefer...

23 July 2016 9,523 5 View

Is there a good anti-NDRG1 antibody for use in mouse liver (or other tissues)?

I'm measuring p-NDRG1 and NDRG1 in mouse tissues. While I have good confidence in my p-NDRG1 antibody (Cell Signalling #5482), I have little confidence in my total NDRG1 antibody (Cell Signalling...

27 April 2016 5,845 1 View

What is the optimal polyacrimide concentration to separate p67 and p64 c-Myc by SDS-PAGE?

I want to resolve and separate p67 and p64 c-Myc protein by SDS-PAGE to recapitulate the below shown figure from the below linked paper. In the paper they used 10% polyacrylamide to separate p67...

07 March 2016 6,511 3 View

Loading Controls for Control vs Dwarf Mouse Livers? Discrepancy in Actin, Tubulin GAPD?

I’ve detected a consistent discrepancy in the apparent expression of Beta Actin and Beta Tubulin in the livers of Snell dwarf mice and controls. This discrepancy exists even after ensuring that...

19 February 2016 4,262 4 View

Poisoning cDNA kit (iScript), exceeding linear range?

I've been having some issues with non-linearity with the Biorad iScript cDNA synthesis kit that I have been trying to troubleshoot.This same problem affects other kits, though perhaps not as...

16 February 2016 902 3 View