Has anyone ever successfully used mouse serum as the sample in a Western blot?
In this case, could you give me some tips? I have home-brew mice polyclonal sera and I tried to check it in WB. Using my normal protocol I have no bands.
In STATISTICA 13.3, I used the Experimental Design Builder under the Design of Experiments tab to generate a design matrix for an asymmetrical factorial design (1443) with 12 experiments. However,...
29 July 2024 4,155 2 View
Much is said about AI and the Communication field. Some say that Communications would very much benefit from that intersection. But specifically which areas would benefit from AI? Could it be a...
11 July 2024 5,335 4 View
Hi everyone! So I am trying to concentrate my recombinant proteins in order to make some assays with them, but I need a "considerable" concentration of each. One of my proteins is around 9kDa and...
08 May 2024 5,418 2 View
Dear all, My question is addresses to reliability regards how reviewers or journal editors would take it when they realize that the methodology of a paper was mainly done through this software. I...
15 April 2024 9,305 0 View
Does anyone know what is the refractive index of MoSi2 and in what medium it can be performed (distilled water or ethyl alcohol)? I pretend to perform a particle-size distribution analysis by...
23 March 2024 6,736 1 View
Does anyone know where to purchase research-grade Galleria mellonella in Europe? I only find the TruLarv from BioSystems Technology, but it seems to be down... Thank you!
11 March 2024 4,800 2 View
I am conducting research on the performance of primary school teachers who use AI, and I have not been able to find a validated instrument that fits this situation. I would like to hear your...
22 February 2024 6,052 4 View
Is is possible that Cretaceous fish, notosuchians, lepidosaurs, or other vertebrates have a morphology that might be confused with "spoon-shaped" sauropod teeth? I have seen a fossil isolated...
08 December 2023 7,040 2 View
I would need two JCPDS cards: 1. BaO (No. 26-0178) 2. Ba(OH)2 (No. 026-0154) I would appreciate a lot if someone could send me both cards... I am a bit desperate. Thanks again for your time!
08 October 2023 4,324 4 View
I am currently working on enhancing the H4rmony dataset, which aims to fine-tune Large Language Models in AI to align them with ecolinguistic values. I'm seeking this classification to assist in...
27 September 2023 972 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View