Jorge Levican Asenjo

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Questions related from Jorge Levican Asenjo

Western blot of mice sera?

Has anyone ever successfully used mouse serum as the sample in a Western blot? In this case, could you give me some tips? I have home-brew mice polyclonal sera and I tried to check it in WB. Using...

24 March 2023 1,652 0 View

N-Glycosylation in silico?

Please anyone can help me to generate a N-glycosylated molecule from a PDB file? Thanks

06 September 2021 4,762 3 View

How to incorporate N-glycosylation in PyMol model??

Hello, Does anyone know how to incorporate (in silico) an N-glycosylation structure in a PyMol modeled protein?

27 January 2021 4,979 1 View

Cytoplasmic recombinant protein purification?

Hello, Any one have a protocol for disrupt cells and obtain cytoplasmic proteins ready to load in HisTrap HP columns? I'm using transient transfection of HEK2913T for recombinant protein...

01 October 2019 3,616 3 View

Are Platinum™ Taq DNA Polymerase High Fidelity (invitrogen) compatible with pGEM easy vector ligation?

Any one knows if the Platinum™ Taq DNA Polymerase High Fidelity (invitrogen) are compatible with pGEM easy vector ligation? Thanks

22 March 2019 1,381 2 View