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Questions related from Jorge Levican Asenjo
Has anyone ever successfully used mouse serum as the sample in a Western blot? In this case, could you give me some tips? I have home-brew mice polyclonal sera and I tried to check it in WB. Using...
24 March 2023 1,701 0 View
Please anyone can help me to generate a N-glycosylated molecule from a PDB file? Thanks
06 September 2021 4,796 3 View
Hello, Does anyone know how to incorporate (in silico) an N-glycosylation structure in a PyMol modeled protein?
27 January 2021 5,007 1 View
Hello, Any one knows how to prepare Phosphate Buffer for binding and elution in His Trap HP purification protocol. At the moment I had used His Buffer Kit and it works well, but now I have to...
02 October 2019 9,740 4 View
Hello, Any one have a protocol for disrupt cells and obtain cytoplasmic proteins ready to load in HisTrap HP columns? I'm using transient transfection of HEK2913T for recombinant protein...
01 October 2019 3,669 3 View
Any one knows if the Platinum™ Taq DNA Polymerase High Fidelity (invitrogen) are compatible with pGEM easy vector ligation? Thanks
22 March 2019 1,414 2 View
Hello, I 'am collecting information to begin a study about the oxidative stress in the generation of colorectal tumors. I would like to begin with an in vitro cellular model, with some non...
01 July 2017 9,079 2 View
