Western blot of HeLa and Vero cells using phospho-Akt (ser473) antibody show double bands at around 70 Kda?

More Assim Verma's questions See All

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

How to estimate the extent to which the FE domain should be meshed with explicit elements in Explicit-Implicit Time integration procedure?

I am developing a MATLAB code for shock problem and want to divide the FE domain into explicit and implicit elements (region of domain at vicinity to shock is meshed into explicit elements and...

15 January 2021 1,968 2 View

How to adjust a covariate in meta-analysis?

Suppose I am meta-analyzing proportions using their logit transformations. We did a meta-regression of the covariates age, gender, and disease severity score in studies and get respective beta...

11 January 2021 9,409 4 View

Is it possible that first waiting time decreases and then after a certain point waiting time start increasing?

The sevice rate is fixed and the arrival rate is changing. As I am plotting the graph of arrival rate with respect to waiting time in queue.

22 December 2020 2,455 1 View

Which gas is used for Bunsen burner in ASTM D6413 Vertical Flame Test?

I'm trying to make a Vertical Flame Test apparatus for flammability test of PVC based leather. I'm not sure about the type of gas which is to be used for generating the flame using bunsen burner.

09 November 2020 1,808 1 View

How does culture impact our ability to learn?

I am working this area, please inform some web resource that will help me.

14 October 2020 9,107 7 View

How to produce ROC/AUC curve using various groundwater vulnerability method for comparative analysis?

ROC/AUC Vulnerability Groundwater Machine learning

11 October 2020 9,186 3 View

When to add H2DCFDA dye before or after treatment and why?

When to add ROS generation binding dye H2DCFDA, before or after treatment and why?

07 September 2020 4,962 3 View

Poisson's ratio of nano hydroxyapatite?

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30 June 2020 7,704 5 View

Why my steel is not showing the RF values when being loaded in modelling of CFST on abaqus ?

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27 April 2020 6,536 13 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

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03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

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02 March 2021 10,003 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

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Help request in CST microwave?

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02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

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01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

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01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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How does an antibody have full intact antibody + half antibody?

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28 February 2021 1,577 1 View

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RIA. How to measure?

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28 February 2021 2,133 3 View

Julie Ann Dougherty

have you tried probing for normal AKT? Is your antibody pan-AKT? as there are multiple AKT isomers. have you already optimized the antibody and included a positive control on your blot to ensure it has worked?

Assim Verma

PAN Akt is working fine and band appears at 60 KDa. What can be used for positive control in case of p-Akt?