Western Blot of Bcl-2

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Abel Tan

Bcl2 dimerises upon apotopsis.Maybe the band you see is a dimer?

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1350923/

Are you using native or denaturing Western Blot?

Goodwin G. Jinesh

https://www.researchgate.net/publication/292615481_Mitochondrial_oligomers_boost_glycolysis_in_cancer_stem_cells_to_facilitate_blebbishield-mediated_transformation_after_apoptosis

See Figure 3e. Is the band is around 60-72 kDa?

Article Mitochondrial oligomers boost glycolysis in cancer stem cell...

Sadia Kanwal

Abl Tan.....i am using running the denaturing WB....and if it is because of apoptosis ....why its appearing in the control sample?

Goodwin its appearing at aroud 28kDa

It may be phosphorylation then..!! BCL2-Ser 70 phosphorylation regulates the survival function (http://www.nature.com/leu/journal/v15/n4/full/2402090a.html) hence this can happen in your control cells too.

many thanks sir...

Quan Qiu

Have you solved this problem?