Foxp3 immunoflurescence protocol ?

Hello guys, I want to stain for Foxp3 transcription factor by immunoflurescence in paraffin embedded mouse tissues. What permeabilization solution i should use and for how long ? also is it...

10 November 2017 5,243 1 View

Any experience in staining HMGB1 for flowcytometry ?

Hello,I was wondering if anyone has stained HMGB1 for flow cytometry before? I will need a recommendation for a good antibody and if possible the staining protocol.It will be good also if you can...

08 September 2017 4,675 4 View

How to check the redox state of a protein in the conditioned culture media

Hi,I was wondering if anyone knows an assay or an easy way in order to check the redox state of a protein that is secreted by the cells in the culture media?

05 June 2017 1,452 1 View

What assays are used to determine the redox state of HMGB1 ?

Hello guys,I wonder if there is any assay that could be used to determine the redox state of HMGB1 protein released in culture media ? your answers will be greatly appreciated

31 December 2016 7,050 3 View

How to dissect the inguinal lymph nodes in mice ?

Hello guys,I wonder if someone can provide me with a link for a good video of murine inguinal lymph nodes dissection as i couldn't find any on Youtube ?

31 December 2016 9,704 3 View

I am focusing on HMGB1 protein in my research and I want to find a way to detect HMGB1 in serum of mice. Can I use the EMSA to detect HMGB1 in serum?

EMSA to detect HMGB1 in murine serum.

09 October 2016 1,156 3 View

I got very few cells when trying to count cells on hemocytometer?

08 September 2016 4,183 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View