Western blot antibodies to be used in-vivo for protein neutralization ?

could antibodies intended for applications such as Western blot be used for in-vivo neutralization of the protein ? Thanks

02 March 2018 3,671 3 View

Foxp3 immunoflurescence protocol ?

Hello guys, I want to stain for Foxp3 transcription factor by immunoflurescence in paraffin embedded mouse tissues. What permeabilization solution i should use and for how long ? also is it...

10 November 2017 5,243 1 View

Any experience in staining HMGB1 for flowcytometry ?

Hello,I was wondering if anyone has stained HMGB1 for flow cytometry before? I will need a recommendation for a good antibody and if possible the staining protocol.It will be good also if you can...

08 September 2017 4,675 4 View

How to check the redox state of a protein in the conditioned culture media

Hi,I was wondering if anyone knows an assay or an easy way in order to check the redox state of a protein that is secreted by the cells in the culture media?

05 June 2017 1,452 1 View

What assays are used to determine the redox state of HMGB1 ?

Hello guys,I wonder if there is any assay that could be used to determine the redox state of HMGB1 protein released in culture media ? your answers will be greatly appreciated

31 December 2016 7,050 3 View

I am focusing on HMGB1 protein in my research and I want to find a way to detect HMGB1 in serum of mice. Can I use the EMSA to detect HMGB1 in serum?

EMSA to detect HMGB1 in murine serum.

09 October 2016 1,156 3 View

I got very few cells when trying to count cells on hemocytometer?

08 September 2016 4,183 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View