Mina Ayoub

8 Questions 6 Answers 0 Followers

Questions related from Mina Ayoub

Western blot antibodies to be used in-vivo for protein neutralization ?

could antibodies intended for applications such as Western blot be used for in-vivo neutralization of the protein ? Thanks

03 March 2018 3,700 3 View

Foxp3 immunoflurescence protocol ?

Hello guys, I want to stain for Foxp3 transcription factor by immunoflurescence in paraffin embedded mouse tissues. What permeabilization solution i should use and for how long ? also is it...

11 November 2017 5,274 1 View

Any experience in staining HMGB1 for flowcytometry ?

Hello,I was wondering if anyone has stained HMGB1 for flow cytometry before? I will need a recommendation for a good antibody and if possible the staining protocol.It will be good also if you can...

09 September 2017 4,705 4 View

How to check the redox state of a protein in the conditioned culture media

Hi,I was wondering if anyone knows an assay or an easy way in order to check the redox state of a protein that is secreted by the cells in the culture media?

06 June 2017 1,514 1 View

How to dissect the inguinal lymph nodes in mice ?

Hello guys,I wonder if someone can provide me with a link for a good video of murine inguinal lymph nodes dissection as i couldn't find any on Youtube ?

01 January 2017 9,739 3 View

What assays are used to determine the redox state of HMGB1 ?

Hello guys,I wonder if there is any assay that could be used to determine the redox state of HMGB1 protein released in culture media ? your answers will be greatly appreciated

01 January 2017 7,076 3 View

I am focusing on HMGB1 protein in my research and I want to find a way to detect HMGB1 in serum of mice. Can I use the EMSA to detect HMGB1 in serum?

EMSA to detect HMGB1 in murine serum.

10 October 2016 1,184 3 View

I got very few cells when trying to count cells on hemocytometer?

I was trying to freeze some cells. my cells were like 80% confluent in the flask and I trypsinized them, tried as much as I can to avoid clumpes by drawing cells up and down several times and then...

09 September 2016 4,212 3 View