Western blot antibodies to be used in-vivo for protein neutralization ?

could antibodies intended for applications such as Western blot be used for in-vivo neutralization of the protein ? Thanks

02 March 2018 3,671 3 View

Foxp3 immunoflurescence protocol ?

Hello guys, I want to stain for Foxp3 transcription factor by immunoflurescence in paraffin embedded mouse tissues. What permeabilization solution i should use and for how long ? also is it...

10 November 2017 5,243 1 View

Any experience in staining HMGB1 for flowcytometry ?

Hello,I was wondering if anyone has stained HMGB1 for flow cytometry before? I will need a recommendation for a good antibody and if possible the staining protocol.It will be good also if you can...

08 September 2017 4,675 4 View

How to check the redox state of a protein in the conditioned culture media

Hi,I was wondering if anyone knows an assay or an easy way in order to check the redox state of a protein that is secreted by the cells in the culture media?

05 June 2017 1,452 1 View

What assays are used to determine the redox state of HMGB1 ?

Hello guys,I wonder if there is any assay that could be used to determine the redox state of HMGB1 protein released in culture media ? your answers will be greatly appreciated

31 December 2016 7,050 3 View

How to dissect the inguinal lymph nodes in mice ?

Hello guys,I wonder if someone can provide me with a link for a good video of murine inguinal lymph nodes dissection as i couldn't find any on Youtube ?

31 December 2016 9,704 3 View

I got very few cells when trying to count cells on hemocytometer?

08 September 2016 4,183 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View