Western blot antibodies to be used in-vivo for protein neutralization ?

could antibodies intended for applications such as Western blot be used for in-vivo neutralization of the protein ? Thanks

02 March 2018 3,671 3 View

Foxp3 immunoflurescence protocol ?

Hello guys, I want to stain for Foxp3 transcription factor by immunoflurescence in paraffin embedded mouse tissues. What permeabilization solution i should use and for how long ? also is it...

10 November 2017 5,243 1 View

Any experience in staining HMGB1 for flowcytometry ?

Hello,I was wondering if anyone has stained HMGB1 for flow cytometry before? I will need a recommendation for a good antibody and if possible the staining protocol.It will be good also if you can...

08 September 2017 4,675 4 View

What assays are used to determine the redox state of HMGB1 ?

Hello guys,I wonder if there is any assay that could be used to determine the redox state of HMGB1 protein released in culture media ? your answers will be greatly appreciated

31 December 2016 7,050 3 View

How to dissect the inguinal lymph nodes in mice ?

Hello guys,I wonder if someone can provide me with a link for a good video of murine inguinal lymph nodes dissection as i couldn't find any on Youtube ?

31 December 2016 9,704 3 View

I am focusing on HMGB1 protein in my research and I want to find a way to detect HMGB1 in serum of mice. Can I use the EMSA to detect HMGB1 in serum?

EMSA to detect HMGB1 in murine serum.

09 October 2016 1,156 3 View

I got very few cells when trying to count cells on hemocytometer?

08 September 2016 4,183 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View