Weak HepG2 cells? - FAQS.TIPS

More Sara Abdelghany's questions See All

Could someone please provide a list of journals that accept the application of methodologies in nature-based solutions?

I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.

23 July 2024 6,444 2 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Cell proliferation assays suggestions?

What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.

21 July 2024 1,554 2 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

How to make chocolate milk ?

we want to produce chocolate milk industrally .Dose anyone have a specific stabilizer formula for this pourpose?

10 July 2024 3,791 1 View

What is your understanding of Precision Medicine?

This forthcoming study aims to gauge public awareness of Precision Medicine and explore how AI and ML technologies can enhance healthcare outcomes. Please consider contributing to this research by...

12 May 2024 7,554 0 View

Efects from trauma in vitiligo?

is there anay resarch about how psychological trauma affects vitiligo?

10 May 2024 5,976 0 View

Matlab codes on the beamforming technique used in intellegant reflective surfaces technology?

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02 May 2024 3,957 1 View

Could you please anyone help me to rectify the following error in my gaussian input file?

When I specify the number of processors = 4, my calculation works fine. But when I want to specify the number of processors more than four, the program gives: "Will use up to 8 processors via...

30 April 2024 3,071 1 View

Sanger sequencing problems?

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29 April 2024 4,613 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

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04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Emmanuel Datan

Hi Sara,

I've cultured HepG2 and I know what your going through. I struggled in one lab with HepG2 but was able to grow them in another. I tweaked with the media after reading about growth conditions. What media are you using?

-ED

Sara Abdelghany

Hi Emmanuel,

I'm currently using RPMI (5%FCS).

Any tips?

Thank you

Try using DMEM 10% FCS 1% PS. If they look different with RPMI can u please let me know? Overall, DMEM makes them happy.