We have used mobio kit for extracting dna from soil but with little success, any suggestions?

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Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Which filtration method to go for run off water from dirty solar panels to be used again?

We are working on a robot that cleans solar panels using fresh water supply and a rotating brush. We are trying to conserve as much water at possible by recycling the dirty water that is collected...

28 July 2024 5,778 2 View

When making abraxane with Nab tech, why is 9:1 Chloroform&EtOH used as solvents?

Is it the best optimization ratio based on experiments?

22 July 2024 8,170 0 View

Can diamond be grown using molecular beam epitaxy?

22 July 2024 9,755 2 View

How can I use LabVIEW to control of an ethernet-based driver?

Hello everyone, I am new to LabVIEW, right now we need to control the movement of an ethernet-based stage drive. We got the sample code from a collaborator but their instrument is USB-based, so we...

11 July 2024 5,385 2 View

Systematic review meta-analysis paper?

Hi everybody, We are trying to write a systematic review meta-analysis paper. But I could find 19 references. I think 19 references are not enough to do a meta-analysis section and it is better to...

10 July 2024 5,490 5 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

Adding isopropanol directly to eluted RNA samples?

I have my RNA samples eluted in nuclease-free water. But now I want to store it for a longer period. Can I add isopropanol directly to my samples to store it without doing a second round of TriZol...

23 June 2024 2,576 4 View

HI, How to find phase response in CST?

I'm simulating a metamaterial unit cell. I want to plot Frequency Vs Phase Response. Also I want to extract permeability and permittivity. Kindly share the steps.

23 June 2024 3,446 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View