We developed an ELISA array for the detection of autoimmune liver disease related antibodies in our experiment. the identified results from ELISA array for M2, SP100, GP210, RO-52 positive sera are agreement with the results from EUROIMMUN KITS, but the results for LC-1 are obviously different. Many "positive" sera identified by ELISA array could not be identified by EUROIMMUN KITS. Beside a possible cause of different antigen used, any other possibility?
Hello Yongjin,
Is it possible that your LC-1 is more sensitive or has a lower limit of detection compared to the KIT? You could confirm this by blotting. If the positive samples from your ELISA are binding to the desired protein, this could be the case and you should titrate some LC-1 and compare the results with each kit. Then you could determine if your ELISA had a lower limit of detection. If there was non specific binding, then the cause of the false positives needs further investigation.
Keep in mind autoimmune liver diseases may have high titers of anti-albumin IgG. A sensitve assay can detect them, Is your solid matrix coated with hu or Bovine albumin/
is your buffer contain albumin? Check all and revalidate.
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