I am working with PRP for platelet isolation. Does checking the efficacy of a drug in modulating platelet function require Washed platelet or PRP method can be useful?
I am trying to pulldown my GFP fused protein of interest using ChromoTek GFP-Trap® Magnetic Agarose. My protein is not binding to the beads. Expression of the GFP-fusion protein has been...
02 June 2024 8,541 0 View
Hello everyone, One can obtain the canonical HF orbitals and localize them by Pipek, Ruedenberg or Boys method to obtain Localized Molecular Orbitals (LMO). One can perform CCSD calculations with...
07 December 2023 3,294 0 View
I have been using PySCF to obtain the two-particle density matrix from a ccsd(t) calculation. However, the calculation is not so memory efficient. But, one can obtain, store ,and manipulate the...
23 October 2023 2,576 0 View
Can anyone help sharing a robust method of sulfonation for the last step of Alexa488 synthesis? It seems quite tricky. I appreciate your help of any suggestion.
17 September 2023 3,200 0 View
Dear All, I am going to use NGA-east attenuation relationship for a work. While I am using the NGA Atkinson Boore attenuation relationship for hard rock site ( Vs=3000 m/sec) an 1.5 time...
03 September 2023 916 1 View
I'm Eagerly waiting to get a chance in the research field image processing
13 June 2023 8,365 5 View
Here, I have attached an out video of an delamination problem. Here, it is noticed that many elements are deleted at a time. But not able to figure out the reason. Any lead in this regards are...
19 October 2022 5,117 1 View
We have seen and observed how cloud can be used to host unlimited data. Is there any possible method that the RAM of a computer can be connected to a cloud service so that computers do not run out...
11 October 2022 9,982 7 View
In am going to perform PSHA analysis by gridded seismicity approach in R-Crisis. But during compilation, I am getting following errors. "bounding boxes of gridded seismicity and geometry are not...
13 September 2022 6,044 1 View
Any existing dataset and R code available for multi-covariate PCA, mostly dealing with different aspects of CT images? Any paper, repository or help will be appreciated.
07 February 2022 10,003 2 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View