Propanna Bandyopadhyay

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Questions related from Propanna Bandyopadhyay

How to stain rodent platelets with Mitotracker Deep Red?

I have been trying to stain platelets with mitotracker but there is no proper staining. Can someone please help? Protocol followed: 1. Addition of 1.2ul Mitotracker in 10ml Opti-MEM solution to...

14 January 2022 3,091 0 View

What is the reason behind blotches in western blots?

I am working with platelets and getting blotches whenever I am running a gel. Why is it so? How can I resolve this problem? I am attaching my Coomassie gel image as a reference.

19 August 2021 7,117 2 View

How to solubilize lyophilized powder of Thrombin from Human Plasma?

I have Sigma's product Thrombin from Human Plasma in lyophilized powder form, 100 units. I need to make 1unit/ml solution for experimental purpose. Thanks!!!

23 December 2020 2,003 3 View

Washed Platelets or Platelet rich plasma?

I am working with PRP for platelet isolation. Does checking the efficacy of a drug in modulating platelet function require Washed platelet or PRP method can be useful?

22 September 2020 2,136 0 View

How to store rodent platelets?

Currently, I am isolating the platelets from Wistar rats (female) by retro-orbital puncture & using ACD buffer as an anticoagulant. Can someone please suggest me the storage conditions that...

22 September 2020 2,291 0 View