Which software (3D simulation)gives optimized solution for modelling the system?

I am doing research on wireless power transfer. Anyone suggest me please

11 June 2020 8,028 8 View

Which stata commands can i use to generate the relative index of inequality?

I am using a relative index of inequality to assess health inequalities yin unmet need in family planning. Whereas I understand the steps to calculate this I am finding trouble with...

25 March 2020 4,206 3 View

Why does the slope looks like in vii7 (realtime)?

The test is for detecting the direct qualitative for Chlamydia trachomatis and Neisseria gonorrhoea. We use FAM (CT), JOE (NG) reporter and CY5 (beta for control). Results are reportable after we...

07 June 2017 9,398 2 View

Is there any tool to find structural similarity percentage of two given proteins?

22 May 2017 1,865 3 View

How to transport rprotein from E.coli? frozen,glycerol,lyopilized which is stable without protein denaturation?

how to transport rprotein from E.coli? frozen,glycerol,lyopilized which is stable without protein denaturation?

22 January 2017 3,320 3 View

Any idea where I could get Nicotiana tabacum cv. BY-2 seeds?

Thank you

30 November 2016 8,889 3 View

How can one determine the variance that arose in a study?

04 May 2016 5,570 2 View

When comparing two methods, is it adequate to use ANOVA or do I need to assess agreement as suggested by Bland and Altman?

I am comparing two methods of determining the strain of the heart. I have used one way ANOVA, but looking at past literature that compared methods of determining strain, they use Bland Altman...

02 May 2016 3,413 3 View

Can someone suggest me a good basic learning material for Dynamic Mechanical Analysis done on polymer composites

28 March 2016 8,094 8 View

How do one teach someone to understand the global and local alignments to a person who is new to bioinformatics?

How can one understand the sequence alignment in a more practical way based on the local and global alignments.

11 February 2016 3,141 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View