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Anyone has experience with a truly permeable DNA stain that will not be adsorbed in plant cell walls? We work on Arabidopsis roots and we tested dyes that are membrane permeable in animal cells...
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Does anybody have experience with GFP fluorescence preservation following aldehyde or other kind of fixation? In our hands formaldehyde fixation (from freshly hydrolyzed 4% paraformaldehyde),...
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Heavy background on pvdf membranes blocked with 5% skimmed milk and probed with affinity purified rabbit pAbs I am probing plant MAPK species using commercially available antibodies from Sigma....
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