5 Questions 42 Answers 0 Followers
Questions related from George Komis
Anyone has experience with a truly permeable DNA stain that will not be adsorbed in plant cell walls? We work on Arabidopsis roots and we tested dyes that are membrane permeable in animal cells...
28 November 2015 5,459 6 View
I am looking for efficient kits for Co-IP using mouse and rabbit IgGs. Effectively co-IP output should be minimally contaminated with IgG heavy/light chains. Any idea?
19 September 2014 9,235 9 View
Does anybody have experience with GFP fluorescence preservation following aldehyde or other kind of fixation? In our hands formaldehyde fixation (from freshly hydrolyzed 4% paraformaldehyde),...
09 September 2012 9,756 88 View
Can anybody provide advice on preservatives for blocking solutions or antibody diluents other than sodium azide? I normally use HRP based chemiluminescence detection and I should avoid sodium azide.
13 July 2012 1,435 12 View
Heavy background on pvdf membranes blocked with 5% skimmed milk and probed with affinity purified rabbit pAbs I am probing plant MAPK species using commercially available antibodies from Sigma....
20 June 2012 2,890 7 View