Vinculin primer sequence for Reverse transcription PCR (RT-PCR)?

More Udesh Dhawan's questions See All

TGF-Beta R1 inhibitor in Osteosarcoma?

Dear fellow researchers: I am working on osteosarcoma cells (U2OS) and am looking for ways to block TGF-Beta signaling pathway (towards Oct-4). Has anyone tried anything related to this...

07 August 2019 4,253 6 View

How to prepare 1.6% chromic acid?

Dear fellow researchers: I need to prepare 1.6% chromic acid for the purpose of AAO etching. The amount of information available online to make chromic acid is overwhelming. I also could not find...

09 October 2018 627 2 View

Cells not uniformly stained in FITC-Phalloidin?

Dear fellow researchers: Ive been doing this experiments for a while but recently I've observed that not all of my cells are getting stained with FITC-Phalloidin. Can anyone suggest possible...

08 September 2018 6,120 9 View

Recommended company to buy TGF-B1 antibody for IF?

Dear fellow researchers, I want to stain TGF-B1 in the cells. I haven't done this in the past. If some has, can anyone recommend a good primary Ab company (tried and tested) with good specific...

07 August 2018 6,637 5 View

No title

Dear Fellow Researchers, I am working on the anodization process and for a brief period of time (3 minutes), I need to achieve and maintain the temperature of the solution (oxalic acid) between...

07 August 2018 8,303 2 View

Why DC Power supply automatically switches from CV to CC while Anodization?

Dear fellow scientists: I am working on fabrication of nonporous Anodised Aluminum Oxide. I am performing the electrolysis in 0.3M oxalic acid at 40V (CV mode in the power supply). However,...

07 August 2018 7,040 6 View

Why the Binding affinity of organic compound with different chirality with FBS is different?

Dear all, I have an organic compound with the difference in chirality. The binding of R-chiralty with Fetal Bovine Serum (FBS) is lower than the binding of S-Chirality with FBS. While I have...

02 March 2018 9,040 12 View

How to perform SRB Assay for cells on opaque substrate?

Dear fellow researchers, I am aware of the SRB assay for analyzing viability of cells cultured directly on-to culture dishes. However, my cells are growing on opaque silicon substrates and I want...

02 March 2018 3,593 3 View

How to measure cellular adhesion to a substrate?

Dear fellow researchers, I'll be running some experiments where I will have to analyze the cellular adhesion to different substrates. Can someone recommend a specific technique to quantify the...

31 December 2017 766 9 View

Why study expression of phosphorylated genes?

Dear all, I have seen in many research papers that scientists study the expression of a gene in the modified stated as well as in the phosphorylated state after a specific treatment. Can anyone...

06 July 2017 5,166 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

David Müller

Hi Udesh,

Unfortunately I don't have these primers in my pocket. However, I can advise you on the right software to use for primer design.

Primerquest from IDT (integrated dna technologies) is the best to me, as I never had melting curves issue or genomic dna amplification.

Good luck !

Udesh Dhawan

Hi David! That should suffice. I'll see what I can do with that. Thank you!

Human