Any one familiar with SOFC/SOEC simulations using Ansys Fluent ?

23 August 2020 8,231 3 View

Adjust annealing temperature or additives to enhance specificity of PCR?

Dear All, I have a very basic question about the PCR/RT-PCR/qPCR. The PCR primers and TaqMan probes are designed such that the primers/probe connot bind to the mismatched site but only...

15 June 2020 4,926 3 View

Crystallization of PLLA?

07 June 2020 2,395 6 View

How to define grain boundary hardness?

08 March 2020 3,388 11 View

Will the TaqMan probe length affect the fidelity of the qPCR assay?

Dear all, I am learning to design the qPCR assays recently. But I just don't know why my negative controls always give positive signal( I have changed my design twice). I checked the formation of...

07 October 2019 4,968 3 View

Effect of mRNA secondary structure on reverse transcription?

Dear Sir/ Madam, I am designing a primer for microRNA reverse transcription. But some microRNAs like hsp-mir-21-5p has a strong secondary structure. It forms a stable hairpin. Can anyone please...

08 September 2019 8,283 3 View

How to deal with the nonlinearity in electrical tomography?

The electrical tomography can be utilized to calculate the solid concentration or gas holdup in aqueous based multiphase flows. However, there usually exists a strong nonlinear relationship...

16 May 2019 197 1 View

How many base-pairs can be added to the pSin vector?

Recently I decide to add a 5.5 kb fragment to pSin vector will it affects the efficiency of virus packaging?

20 March 2019 9,786 2 View

How can I deal with the non-specific binding in XIST-RNA-FISH?

I'm trying to detect the XIST-RNA expression in Breast cancer cells using the Biotin and Digoxin probes. However, there are always non-specific bindings in my results. I have added RNase-inhibitor...

18 March 2019 3,761 1 View

Any UV light absorbing material at 193-248 nm?

Would like to coat the alloy surface with non-organic material to absorb the UV light at 193-248 nm. Please recommend some UV absorbing material. Thanks~

16 November 2018 1,466 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View