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Questions related from Binbin Cui
Dear All, I am doing IF over mouse spleen recently. But when I sectioned the tissue into slices, I met some problems. I fixed the mouse whole spleen with 4% PFA in PBS at 4C for 5, 8, and 18...
05 September 2024 5,826 0 View
Dear All, I am doing ligation of the padlock probe. The efficiency is pretty good (more than 90%). But I have a very simple question about this process. As shown in the following figure, in...
09 November 2021 9,485 1 View
Dear All, I am doing a ligation assited RCA. I have a padlock probe whose two ends are complementary with a target molecule in a contatenated way. I use HiFi Ligase to ligate the padlock probe....
26 March 2021 7,875 5 View
Dear All, I am doing one-step reverse transcription PCR in droplet, which is based on TaqMan probe. About half a year ago, everything works well. But recenly, the ddPCR starts generating some odd...
11 October 2020 638 3 View
Dear All, I have a very basic question about the PCR/RT-PCR/qPCR. The PCR primers and TaqMan probes are designed such that the primers/probe connot bind to the mismatched site but only...
16 June 2020 5,103 3 View
Dear all I am doing a droplet digital PCR with fluoro-oil like FC-40 and HFE-7500 supplemented by 5% EA surfactant(RAN). For enzymes like the HS Taq(NEB), I could get the positive droplets(all...
28 April 2020 255 0 View
Dear all, I am learning to design the qPCR assays recently. But I just don't know why my negative controls always give positive signal( I have changed my design twice). I checked the formation of...
08 October 2019 5,153 3 View
Dear Sir/ Madam, I am designing a primer for microRNA reverse transcription. But some microRNAs like hsp-mir-21-5p has a strong secondary structure. It forms a stable hairpin. Can anyone please...
09 September 2019 8,436 3 View
Dear all, I am amplifying a target sequence of 65 bases, which is a cDNA sequence now. For some reasons, my PCR primer can only bind to the target sequence with 11 bases in the first PCR cycle....
31 October 2018 960 7 View
Dear all, I am detecting mRNAs with hairpin molecular beacon. The 5' end of the hairpin oligo is modified with BHQ1 and the 3' end FAM. After I dissolved the hairpin in NF water, I dispensed one...
27 August 2018 4,802 4 View
Usually we break the exosome with lysis buffer(eg, tritonx-100). But I am wondering if we can break the exosome by heating it? Thank a lot for any suggestions and helps!
04 April 2018 524 1 View
I am making droplet with microfluidic chip. The aqueous phase is the acrylamide solution and the carrier oil is HFE-7500 with Fluorosurfactant. In order to catalyze the polymerization of...
24 July 2017 3,993 0 View
can anyone please tell me how to control the droplet size. I am using fluorosurfactant and HFE7500 oil. I only know that I can change the diameter of the droplet by controlling the flow rate. but...
10 July 2017 9,899 27 View
