Universal primer used in detection of wide range of bacteria

01 January 1970 1 4K Report

i have to prepare my own universal primer , i am asking how can do that

One way would be to direct them against conversed regions in the 16S rRNA. An interesting paper on the subject can be read here:

Article Gram Type-Specific Broad-Range PCR Amplification for Rapid D...

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Science method

More Zahid Aziz's questions See All

Is there resistance to natural plant?

We always read in papers and articles that natural plant extracts have antibacterial or in general antimicrobial effects so are these extracts lmpact the habitats of the microbiota of our body and...

01 February 2020 4,033 2 View

Any one tell me plant dye do as intercalating agent?

Intercalating agent any substance which can lodges between dna nucleotides

10 November 2018 2,454 3 View

What is delta G value?

my question related to primer self complementary and its concerning with secondary structure and dimers.

05 June 2017 10,397 3 View

Self complementarity

my question is that what is the maximum limit of self complemtarity of primer which do not impact the pcr amplification and can cause dimer or nonspecific product

05 June 2017 1,926 0 View

Are there proofs that prion might related to errors or defects in protein translation or misfolding?

prion , translation

06 July 2016 5,655 2 View

I ask if i can evaporate ethanol from mixtures by boiling to 78.37 c ?

ethanol, boiling

03 April 2016 1,648 4 View

I am ask the relation ship between androgen level in adult male and brain amyloid lesions ?

crzay cow , amyloid lesions

07 August 2015 6,009 0 View

Iam asking about the adult chronic dehydration and if this state related to demineralized water ?

reverse osmosis , chronic dehydration , demineralised water

07 August 2015 9,004 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View