my question is that what is the maximum limit of self complemtarity of primer which do not impact the pcr amplification and can cause dimer or nonspecific product
We always read in papers and articles that natural plant extracts have antibacterial or in general antimicrobial effects so are these extracts lmpact the habitats of the microbiota of our body and...
01 February 2020 4,033 2 View
Intercalating agent any substance which can lodges between dna nucleotides
10 November 2018 2,454 3 View
my question related to primer self complementary and its concerning with secondary structure and dimers.
05 June 2017 10,397 3 View
prion , translation
06 July 2016 5,655 2 View
ethanol, boiling
03 April 2016 1,648 4 View
crzay cow , amyloid lesions
07 August 2015 6,009 0 View
reverse osmosis , chronic dehydration , demineralised water
07 August 2015 9,004 0 View
i have to prepare my own universal primer , i am asking how can do that
01 January 1970 4,276 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?
31 July 2024 3,479 5 View
What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?
31 July 2024 8,483 8 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View