Dear researchers,

I currently prepare different protein aggregates (monomer, oligomer and protofibril) using methods in literatures and protect them by PICUP before running gels. Because previously I found my irradiation time might be too short, I elongated the irradiation time to 5s and 10s, but I still can't find any useful information after Coomassie colouration. There are no clear bands, only something like shadow. And there are no protofibril bands, whose molecular weight is over 100 kDa.

My gel percentage is 12%, and I use tris-glycine gel. The running voltage is 80V in 40 minutes and 120V in 60 minutes. I wanna know what could this gel tell me, and what I should do to see clear protein bands. Thanks a lot!