Pangmiaomiao Zhang

4 Questions 3 Answers 0 Followers

Questions related from Pangmiaomiao Zhang

Question regarding imaging Ni-His tag proteoliposome under HS-AFM?

Hi all, I'm recently using interaction of Nickel NTA lipid and His-tag protein to make proteoliposomes by simply mix liposomes (DOPC:DOPS:DOGS-Ni-NTA = 3:2:30%) and His tagged proteins at 4...

27 October 2024 2,784 0 View

How to cleave the glycans on SARS-CoV-2 spike proteins?

Hi all, For the imaging of fine structure on the surface of spike proteins, I want to try to cleave glycans on the protein surface. Is there any enzyme/method that can be used to do it without...

28 October 2023 3,836 0 View

Unclear SDS-PAGE protein bands in amyloid β peptide determination?

Dear researchers, I currently prepare different protein aggregates (monomer, oligomer and protofibril) using methods in literatures and protect them by PICUP before running gels. Because...

18 March 2021 7,294 3 View

How to deal with foam producing in amyloid β dissolution?

Hi everyone, I am a master student who currently working with a program detecting amyloid β aggregation, so I need to use Abeta 42. After treating with HFIP and get a peptide film, I want to...

24 February 2021 4,465 3 View