I am using a Nrf2 Antibody (A-10): sc-365949 from santacruz which is a new replaced monoclonal antibody. With this antibody, I am getting an intense single band in my control sample at around 50-60kda.
But after treatment of my drug of interest gives extra clear band at 110 kda. The most interesting thing is with dose treatment of drug the 50-60kda band decreases while at higher drug treatment 110kda intensifies.
Many papers have their different views in Nrf2 molecular weight and true Nrf2 band in western Blot. Here, I just needed to know which band, I should consider for Nrf2 expression and if 50-60kda band is non-specific band, then why it is decreasing with the treatment and if not, why one band is decreasing and other one (110kda) is increasing.
Please help in this regard.