Suchitra Mohanty

4 Questions 7 Answers 0 Followers

Questions related from Suchitra Mohanty

True Nrf2 band in western blot ?

I am using a Nrf2 Antibody (A-10): sc-365949 from santacruz which is a new replaced monoclonal antibody. With this antibody, I am getting an intense single band in my control sample at around...

04 April 2018 779 3 View

What are the markers for iDC derived from human PBMC stimulated with IL-4 and GM-CSF?

I have isolated PBMC from human blood and allowed to adhere to plastic surface for 3 hours and then washed with media to get rid floating cells.Then adherent cells were stimulated with IL-4 and...

01 January 2015 6,642 14 View

How can I normalize the cell number for a cytokine assay?

hello everyone I wanted to do bioplex cytokine assay in a cell line model after transfecting my gene of interest through electroporation method.As electroporation of cells gives lots of cell death...

12 December 2014 3,652 3 View

What can be done to avoid clumps in JSC-1 and HBL-6 cell lines?

We are growing these cells in 10% serum in RPMI media. After one week of sub-culturing these cells, they suddenly started clumping .Can anyone suggest how to avoid these clumps?

05 May 2013 8,518 6 View