hello everyone
I wanted to do bioplex cytokine assay in a cell line model after transfecting my gene of interest through electroporation method.As electroporation of cells gives lots of cell death as well as my gene of interest is also inducing cell death in cell line,here I like to know how to normalize cell numbers in each group for checking and comparing cytokine level.
Thanks everyone for your valuable time.
Hello
That electroporation is a relatively quick and reliable method to transiently modify a number of cell lines as well as a macrophage cell line. and you can use For many purposes, according to the objective researcher , it is electroporation
increases the efficiency and ease of selecting the best promoter able to drive transgene expression .
please read the article in attachment , i hope help you
Good luck
After electroporation, just do a cell count with trypan blue to normalize cell numbers and get an idea of viability.
It is always a good idea to look at viability after something like electroporation because of its known affect on cells.
If experiments are performed with culture supernatents than it is must to standardize the cell viability for a certain time point when viability remains same for different groups
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