Hi!
Does anyone have experience of shipping extracted total RNA at ambient temperatures, for RNA seq;
(i) using DNA/RNAshield using Zymogen? (or recommend an alternate product. RNAstable has been discontinued)
(ii) ethanol precipitated RNA (ethanol, 3M sodium acetate)?
Thanks!
Dear Dania,
GenTegraRNA is the immediate replacement for the discontinued product, RNAstable™.
https://gentegra.com/products/#stabilization
RNA later (Qiagen) is another option. Alternatively you can convert your RNA to cDNA, lyopholize/dry it. We have shipped lyopholized cDNA at ambient temperature.
Hi Mehdi,
I have transported RNA at ambient temperature for sequencing and works good. Firstly, I precipitated with of 0.1 volumes of 3 M sodium acetate (pH 5.2), 2.5
volumes of ethanol and 2 μl of glycogen (10 mg/ml) (ON). Secondly RNA was centrifuged at 14,000 g for 30 min at 4C, washed in 75% ethanol and dried in speed-back. Everything has to be RNAase-free.
Other options are RNA later or RNA Transport.
Good luck!
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