Dania Saeed

4 Questions 3 Answers 0 Followers

Questions related from Dania Saeed

Can we create a heatmap for differentially expressed genes taking contrast between two levels of a factor?

I am looking at treatment as a factor with three levels; baseline, treated, untreated. My dds design included treatment, and genotype. Now i want to focus on the treatment contrast between treated...

06 July 2023 658 2 View

RIN value is greater than 7 but rRNA ratio < 1?

Hi. I extracted total RNA from Mycobacterium tuberculosis (MTB) isolates. The RIN for some of the samples is >7 but rRNA ratio is below 1. Tapestation RNA screen tape was used. I am attaching...

25 December 2022 2,486 2 View

RIN value for RNA seq?

Hi! I am working on extracting RNA from Mycobacterium tuberculosis isolates under drug pressure for RNA seq. Can we use RNA elutes of RIN value ≥ 6 & rRNA 0.7?

17 August 2022 7,137 0 View

Transportation of RNA at ambient temperature?

Hi! Does anyone have experience of shipping extracted total RNA at ambient temperatures, for RNA seq; (i) using DNA/RNAshield using Zymogen? (or recommend an alternate product. RNAstable has...

10 September 2021 6,564 3 View