Transformation issue in cyanobacteria?

26 December 2017 1 6K Report

I am transforming a local isolate of cyanobacteria with a construct as follows: Promoter-eyfp-terminator. After transforming the construct I am only getting the eyfp band and my antibiotic, spectinomycin band and no band with the complete construct primer. Also I am not getting any band with conformational primer which are designed using the NSL1 region. Is it possible that the construct is getting fragmented and the fragments are getting integrated in different parts of the genome? How should I solve such an issue?

Mark Farman

Not enough detail given. However, your reference to "complete construct primer" suggest that you are using a linear fragment for transformation, in which case it's possible that the ends were resected before integration.

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