Does anyone know if sodium azide is cytotoxic to mammalian cells (293, CHO, murine hybridomas) at 0.1-0.2% w/v? Buffers such as MACS buffer seem to include it but I would have thought this would kill the cells?
This can be of assistance.....
Hello Christopher.
Yes, it is. It is a metabolic inhibitor, used as preservative to prevent bacterial growth at 0.002%(w/v). Thus it should be kept at minimal in cells.
Cheers.
Hi Christopher,
It has different degree of toxicity toward mammalian cells, please find more at:
https://www.researchgate.net/post/Can_anyone_provide_any_information_on_the_toxicity_range_of_sodium_azide_in_cell_culture
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